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KMID : 0359320010410030367
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Korean Journal of Veterinary Research
2001 Volume.41 No. 3 p.367 ~ p.371
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Occurrence of postweaning multisystemic wasting syndrome in pigs in Jeju
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신태균/Shin, Tae Kyun
이두식/안미정/강종철/김진희/정경주/강완철/Lee, Du Sik/Ahn, Mee Jung/Kang, Jong Chul/Kim, Jin Hoe/Jeong, Kyong Ju/Kang, Wan Chul
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Abstract
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Multiples PCR and immunohistochemistry were used to detect and differentiate between porcine circovirus (PCV) type-I and the PCV associated with postweaning multisystemic wasting syndrome(PMWS). Unique DNA product to PCV type-Ⅱ was confirmed the some organs including lymph nodes, tonsil and spleen from eight pigs in Jeju by multiplex PCR. In this study, the samples were tested by a multiplex PCR assay to determine the type of PCV in each case; all cases were PCV type-Ⅱ positive. PCV type-Ⅱ was identified not only in typical PMWS cases, but also in field cases submitted with various clinical histories, some of which were not suggestive of PMWS. Immunohistochemically PCV type-Ⅱ antigen wasd detected in macrophage-like cells in the tonsil, liver, lymph nodes and spleen, while some hepatocytes and renal tubular epithelial cells were also positive to the virus. This study suggested that PCV type-Ⅱ is one of the causative agents of PMWS as well as the major type of PCV in the affected pigs in Jeju.
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KEYWORD
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porcine circovirus, polymerase chain reaction, immunohistochemistry
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